CEBPA antibody (pThr230)

Catalog No. ABIN8120293

The Rabbit Polyclonal anti-CEBPA antibody (ABIN8120293) specifically detects CEBPA in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CEBPA antibody (pThr230) (ABIN8120293)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CEBPA Antibody

Binding Specificity: pThr230

Purpose: C/EBP α (phospho Thr230) Antibody

Specificity: Phospho-C/EBP α (T230) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T230.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human C/EBP alpha (phospho Thr230)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: C/EBP alpha

Background:

Synonyms: CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha

Background: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],

Gene Name: CEBPA

Protein Name: CCAAT/enhancer-binding protein alpha

Gene ID: 1050

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation