DDX4 antibody

Catalog No. ABIN8120930

This Rabbit Monoclonal antibody specifically detects DDX4 in WB, ELISA, IHC, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DDX4 antibody (ABIN8120930)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2316

Product Details anti-DDX4 Antibody

Purpose: DDX4 Rabbit mAb

Specificity: This antibody detects endogenous levels of Probable ATP-dependent RNA helicase DDX4 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4

Background:

Synonyms: DDX4,VASA,Probable ATP-dependent RNA helicase DDX4,DEAD box protein 4,Vasa homolog

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) , are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Gene Name: DDX4

Protein Name: Probable ATP-dependent RNA helicase DDX4

Gene ID: 54514

UniProt: Q9NQI0