DFFA antibody (Cleaved-Thr225)

Catalog No. ABIN8121031

The Rabbit Polyclonal anti-DFFA antibody (ABIN8121031) specifically detects DFFA in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DFFA antibody (Cleaved-Thr225) (ABIN8121031)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DFFA Antibody

Binding Specificity: Cleaved-Thr225

Purpose: DFF45 (Cleaved-Thr225) Antibody

Specificity: This antibody detects endogenous levels of Human DFF45 (Cleaved-Thr225, protein was cleaved amino acid sequence between 224-225 )

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human DFF45 (Cleaved-Thr225)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFF45

Background:

Synonyms: DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD)

Background: function:Inhibitor of the caspase-activated DNase (DFF40).,PTM:Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 CIDE-N domain.,subunit:Heterodimer of DFFA and DFFB.,

Gene Name: DFFA DFF1 DFF45 H13

Protein Name: DFF45 (Cleaved-Thr225)

Gene ID: 1676

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis