EEA1 antibody

Catalog No. ABIN8121126

The Rabbit Monoclonal anti-EEA1 antibody is suitable to detect EEA1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and IP.

Quick Overview for EEA1 antibody (ABIN8121126)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1761

Product Details anti-EEA1 Antibody

Purpose: EEA1 Rabbit mAb

Specificity: This antibody detects endogenous levels of EEA1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:100-1:200 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background:

Synonyms: EEA1, ZFYVE2, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

Background: disease:Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases. , domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate. , function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. , similarity:Contains 1 C2H2-type zinc finger. , similarity:Contains 1 FYVE-type zinc finger. , subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. ,

Gene Name: EEA1

Protein Name: EEA1

Gene ID: 8411

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades