EIF2A antibody (pSer51)

Catalog No. ABIN8121299

This Mouse Monoclonal antibody specifically detects EIF2A in WB, IF and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EIF2A antibody (pSer51) (ABIN8121299)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: AB2377

Product Details anti-EIF2A Antibody

Binding Specificity: pSer51

Purpose: eIF2α (Phospho Ser51) mouse mAb

Specificity: This antibody detects endogenous levels of eIF2α (Phospho Ser51) protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthesized phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: eIF2alpha

Background:

Synonyms: Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha) , EIF2S1, EIF2A

Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1) , the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908) , and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. , 1987 [PubMed 2948954]) . [supplied by OMIM, Feb 2010],

Gene Name: EIF2S1

Protein Name: eIF2α

Gene ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly