Fascin antibody (AA 250-350)

Catalog No. ABIN8121393

The Mouse Monoclonal anti-Fascin antibody is suitable to detect Fascin in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for Fascin antibody (AA 250-350) (ABIN8121393)

Target: Fascin (FSCN1)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: AB1541

Product Details anti-Fascin Antibody

Binding Specificity: AA 250-350

Purpose: Fascin mouse mAb

Specificity: The antibody can specifically recognize human Fascin protein. In western blotting of Hela cell lysate, the antibody can label a 55 kDa band corresponding to Fascin, while there is no band detected in Fascin knock out Hela cell lysate.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 250 - 350 of the human Fascin protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: Fascin

Background:

Synonyms: 55 kDa actin bundling protein, 55 kDa actin-bundling protein, Actin bundling protein, actin bundling protein, 55-KD, FAN 1, FAN1, Fascin 1, Fascin actin bundling protein 1, Fascin, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuRatus) , Fascin homolog 1, Fascin, sea urchin, homolog of, 1, Fascin1, FLJ38511, FSCN 1, FSCN1, FSCN1_HUMAN, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like) , Singed drosophila homolog like, Singed like (fascin homolog sea urchin) (Drosophila) , Singed like (fascin homolog sea urchin) , Singed like protein, Singed, drosophila, homolog of, Singed-like protein, SNL, Strongylocentrotus purpuRatus

Background: Fascin is a highly conserved actin bundle protein, which is located in microfilaments and stress fibers involved in microfilament bundle formation. It is mainly expressed in dendritic cells and is a sensitive marker of R-S cells. Lymphocytes, plasma cells and myeloid cells did not express. It can be used to diagnose Hodgkin's lymphoma and Langerhans cell histiocytosis.

Gene Name: FSCN1

Protein Name: Fascin

Gene ID: 6624

UniProt: Q16658