GATA1 antibody (acLys312)

Catalog No. ABIN8121619

The Rabbit Polyclonal anti-GATA1 antibody (ABIN8121619) specifically detects GATA1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GATA1 antibody (acLys312) (ABIN8121619)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GATA1 Antibody

Binding Specificity: acLys312

Purpose: GATA-1 (Acetyl Lys312) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat GATA-1 (Acetyl Lys312)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human GATA-1 (Acetyl Lys312)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA-1

Background:

Synonyms: Erythroid transcription factor (Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein)

Background: disease:Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.,disease:Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050], also called thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. The disease consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced (hemo)globin chain synthesis resembling that of beta-thalassemia minor.,domain:The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.,function:Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.,PTM:Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.,PTM:Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.,similarity:Contains 2 GATA-type zinc fingers.,subunit:Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4, the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner.,tissue specificity:Erythrocytes.,

Gene Name: GATA1 ERYF1 GF1

Protein Name: GATA-1 (Acetyl Lys312)

Gene ID: 2623

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin