HCK antibody (pTyr521)

Catalog No. ABIN8121927

The Rabbit Polyclonal anti-HCK antibody is suitable to detect HCK in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for HCK antibody (pTyr521) (ABIN8121927)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HCK Antibody

Binding Specificity: pTyr521

Purpose: Hck (phospho Tyr521) Antibody

Specificity: Phospho-Hck (Y521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Hck (phospho Tyr521)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: Hck

Background:

Synonyms: HCK, Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck

Background: The protein encoded byThis gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],

Gene Name: HCK

Protein Name: Tyrosine-protein kinase HCK

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling