Histone H2A antibody

Catalog No. ABIN8122005

The Rabbit Monoclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for Histone H2A antibody (ABIN8122005)

Target: Histone H2A

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1615

Product Details anti-Histone H2A Antibody

Purpose: Histone H2A (Ubiquityl Lys119) Rabbit mAb

Specificity: This antibody detects endogenous levels of Histone H2A (Ubiquityl Lys119) protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Histone H2A

Target: Histone H2A

Background:

Synonyms: Histone H2A type 1, H2A. 1, Histone H2A/p,

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21. 3. [provided by RefSeq, Aug 2015],

Gene Name: HIST1H2AG H2AFP, HIST1H2AI H2AFC, HIST1H2AK H2AFD, HIST1H2AL H2AFI, HIST1H2AM H2AFN

Protein Name: Histone H2A (Ubiquityl Lys119)

Gene ID: 8329

UniProt: P0C0S8