Mast Cell Tryptase antibody (AA 200-275)

Catalog No. ABIN8122774

The Mouse Monoclonal anti-Mast Cell Tryptase antibody (Clone AB2346) (ABIN8122774) specifically detects Mast Cell Tryptase in IHC, WB, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Mast Cell Tryptase antibody (AA 200-275) (ABIN8122774)

Target: Mast Cell Tryptase

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mast Cell Tryptase antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB2346

Product Details anti-Mast Cell Tryptase Antibody

Binding Specificity: AA 200-275

Purpose: Mast cell tryptase mouse mAb

Specificity: This antibody detects endogenous levels of Mast cell tryptase protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 200 - 275 of the human Mast cell tryptase protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Mast Cell Tryptase

Target: Mast Cell Tryptase

Background:

Synonyms: alpha II, Lung tryptase, Mast cell alpha II tryptase, Mast cell beta I tryptase, Mast cell protease 7, Mast cell protease II, MCP 7, Pituitary tryptase, Skin tryptase, TPS 1, TPS1, TPS2, TPSAB1, TPSAB1 protein, TPSB1, Tryptase 1, Tryptase alpha 1, tryptase alpha I included, Tryptase alpha II, tryptase alpha II included, tryptase alpha included, tryptase alpha/beta 1, Tryptase beta 1, tryptase beta I included, Tryptase I, tryptase I included, Tryptase III, Tryptase skin

Background: Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13. 3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate gene

Gene Name: TPSAB1

Protein Name: Mast cell tryptase

Gene ID: 7177

UniProt: Q15661