MMP1 antibody (Cleaved-Ile271)

Catalog No. ABIN8122794

The Rabbit Polyclonal anti-MMP1 antibody is suitable to detect MMP1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for MMP1 antibody (Cleaved-Ile271) (ABIN8122794)

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MMP1 Antibody

Binding Specificity: Cleaved-Ile271

Purpose: MMP1 (Cleaved-Ile271) Antibody

Specificity: This antibody detects endogenous levels of Human MMP1 (Cleaved-Ile271, protein was cleaved amino acid sequence between 270-271 )

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human MMP1 (Cleaved-Ile271)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MMP1

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Alternative Name: MMP1

Background:

Synonyms: Interstitial collagenase (EC 3.4.24.7, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]

Background: catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,domain:There are two distinct domains in this protein, the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).,enzyme regulation:Can be activated without removal of the activation peptide.,function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.,online information:Collagenase entry,PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subunit:Interacts with HIV-1 Tat.,

Gene Name: MMP1 CLG

Protein Name: MMP1 (Cleaved-Ile271)

Gene ID: 4312

UniProt: P03956