MITF antibody

Catalog No. ABIN8122919

This Rabbit Monoclonal antibody specifically detects MITF in WB, IHC, IF, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MITF antibody (ABIN8122919)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB1692

Product Details anti-MITF Antibody

Purpose: MiTF Rabbit mAb

Specificity: This antibody detects endogenous levels of MiTF protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:500 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MiTF

Background:

Synonyms: MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

Background: This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

Gene Name: MITF

Protein Name: MiTF

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding