MLH1 antibody (AA 400-500)

Catalog No. ABIN8123089

The Mouse Monoclonal anti-MLH1 antibody (Clone AB1558) (ABIN8123089) specifically detects MLH1 in WB, IHC, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for MLH1 antibody (AA 400-500) (ABIN8123089)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: AB1558

Product Details anti-MLH1 Antibody

Binding Specificity: AA 400-500

Purpose: MutL Protein Homolog 1 (MLH1) mouse mAb

Specificity: This antibody detects endogenous levels of MLH1 protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 400 - 500 of the human MutL Protein Homolog 1 protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:100-1:500 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MutL Protein Homolog 1

Background:

Synonyms: DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1

Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009],

Gene Name: MLH1 COCA2

Protein Name: MutL Protein Homolog 1

Gene ID: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response