MLL/KMT2A antibody (AA 3850-3900)

Catalog No. ABIN8123112

The Rabbit Polyclonal anti-MLL/KMT2A antibody is suitable to detect MLL/KMT2A in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for MLL/KMT2A antibody (AA 3850-3900) (ABIN8123112)

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL/KMT2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MLL/KMT2A Antibody

Binding Specificity: AA 3850-3900

Purpose: MLL Antibody

Specificity: The antibody detects endogenous MLL

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic peptide from human protein at AA range: 3850-3900

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MLL/KMT2A

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Alternative Name: MLL

Background:

Synonyms: Histone-lysine N-methyltransferase MLL (EC 2.1.1.43, ALL-1, CXXC-type zinc finger protein 7, Lysine N-methyltransferase 2A, KMT2A, Trithorax-like protein, Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa, p320), MLL cleavage product C180 (C-terminal cleavage product of 180 kDa, p180)]

Background: This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefS

Gene Name: MLL ALL1 CXXC7 HRX HTRX KMT2A MLL1 TRX1

Protein Name: Histone-lysine N-methyltransferase MLL (EC 2.1.1.43) (ALL-1) (CXXC-type zinc finger protein 7) (Lysine N-methyltransferase 2A) (KMT2A) (Trithorax-like protein) (Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa) (p320)MLL cleavage product C180 (C-terminal cleavage product of 180 kDa) (p180)]

Gene ID: 4297

UniProt: Q03164

Pathways: Warburg Effect