MYLK antibody (pTyr471)

Catalog No. ABIN8123150

This Rabbit Polyclonal antibody specifically detects MYLK in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MYLK antibody (pTyr471) (ABIN8123150)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYLK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MYLK Antibody

Binding Specificity: pTyr471

Purpose: MLCK (Phospho Tyr471) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat MLCK (Phospho Tyr471)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human MLCK (Phospho Tyr471)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: MLCK

Background:

Synonyms: Myosin light chain kinase, smooth muscle (MLCK; smMLCK; EC 2.7.11.18; Kinase-related protein; KRP; Telokin) [Cleaved into: Myosin light chain kinase, smooth muscle, deglutamylated form]

Background: myosin light chain kinase(MYLK) Homo sapiens This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity.This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been

Gene Name: MYLK MLCK MLCK1 MYLK1

Protein Name: MLCK (Phospho Tyr471)

Gene ID: 4638

UniProt: Q15746