NDUFA1 antibody

Catalog No. ABIN8123219

This Rabbit Monoclonal antibody specifically detects NDUFA1 in WB, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for NDUFA1 antibody (ABIN8123219)

Target: NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NDUFA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: AB2254

Product Details anti-NDUFA1 Antibody

Purpose: NDUFA1 Rabbit mAb

Specificity: This antibody detects endogenous levels of NDUFA1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:•100-1:300 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. |

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for NDUFA1

Target: NDUFA1 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1))

Alternative Name: NDUFA1

Background:

Synonyms: NDUFA1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 , Complex I-MWFE, CI-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit,

Background: The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the ", hydrophobic protein", (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008],

Gene Name: NDUFA1

Protein Name: NDUFA1

Gene ID: 4694

UniProt: O15239