Podoplanin antibody (AA 23-100)

Catalog No. ABIN8123830

The Mouse Monoclonal anti-Podoplanin antibody (Clone AB1449) (ABIN8123830) specifically detects Podoplanin in WB, IHC, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for Podoplanin antibody (AA 23-100) (ABIN8123830)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: AB1449

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 23-100

Purpose: Podoplanin mouse mAb

Specificity: The antibody can specifically recognize human Podoplanin protein. In western blotting of U2O2 cell lysate, the antibody can label a 36 kDa band corresponding to Podoplanin.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 23 - 100 of the human Podoplanin protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background:

Synonyms: Aggrus, Glycoprotein 36, Gp36, PA2. 26 antigen, T1-alpha, T1A

Background: Podoplanin, also known as D2-40 or M2A, is a 38 kDa type I transmembrane mucin with multiple O-glycosylation sites. It is highly expressed in lymphatic endothelial cells, but not in blood vessels. In addition, it is also expressed in a variety of cells, such as mesothelial cells and type I alveolar epithelial cells, follicular dendritic cells, reticular cells, ovarian and testicular germ cells. Podoplanin is a very useful marker to distinguish malignant mesothelioma and adenocarcinoma, seminoma and follicular dendritic cell sarcoma. It is also the main choice to show and quantify lymphatic and intravascular tumor infiltration.

Gene Name: PDPN

Protein Name: Podoplanin

Gene ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport