PKC theta antibody (pTyr90)

Catalog No. ABIN8124075

The Rabbit Polyclonal anti-PKC theta antibody is suitable to detect PKC theta in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for PKC theta antibody (pTyr90) (ABIN8124075)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PKC theta Antibody

Binding Specificity: pTyr90

Purpose: PKC θ (phospho Tyr90) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat Phospho PKC θ (Y90)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human Phospho PKC theta (Y90)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PKC theta

Background:

Synonyms: Protein kinase C theta type (EC 2.7.11.13, nPKC-theta)

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites, Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.,PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with TXNL2/PICOT.,tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.,

Gene Name: PRKCQ PRKCT

Protein Name: Phospho PKC θ (Y90)

Gene ID: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling