Pyruvate Dehydrogenase E1 alpha antibody

Catalog No. ABIN8124176

This Rabbit Monoclonal antibody specifically detects Pyruvate Dehydrogenase E1 alpha in WB, IF, ELISA, IP and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Pyruvate Dehydrogenase E1 alpha antibody (ABIN8124176)

Target: Pyruvate Dehydrogenase E1 alpha

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: AB1854

Product Details

Purpose: Pyruvate Dehydrogenase E1 α Rabbit mAb

Specificity: This antibody detects endogenous levels of Pyruvate Dehydrogenase E1 α protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details

Target: Pyruvate Dehydrogenase E1 alpha

Background:

Synonyms: PDHA1, PHE1A, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) , and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1) , dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],

Gene Name: >>Glycolysis / Gluconeogenesis, >>Citrate cycle (TCA cycle) , >>Pyruvate metabolism, >>Metabolic pathways, >>Carbon metabolism, >>HIF-1 signaling pathway, >>Glucagon signaling pathway, >>Central carbon metabolism in cancer, >>Diabetic cardiomyopathy

Protein Name: Pyruvate Dehydrogenase E1 α

Gene ID: 5160

UniProt: P08559