PKM2 antibody (AA 301-350)

Catalog No. ABIN8124183

The Rabbit Polyclonal anti-PKM2 antibody is suitable to detect PKM2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for PKM2 antibody (AA 301-350) (ABIN8124183)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PKM2 Antibody

Binding Specificity: AA 301-350

Purpose: PKM2 Antibody

Specificity: This antibody detects endogenous levels of human PKM2

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PKM2 AA range: 301-350

Isotype: IgG

Application Details

Application Notes: IHC 1:50-200, WB 1:500-2000. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background:

Synonyms: Pyruvate kinase isozymes M1/M2 (EC 2.7.1.40, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58)

Background: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011],

Gene Name: PKM OIP3 PK2 PK3 PKM2

Protein Name: PKM2

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect