RAB5 antibody

Catalog No. ABIN8124212

This Rabbit Monoclonal antibody specifically detects RAB5 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RAB5 antibody (ABIN8124212)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1844

Product Details anti-RAB5 Antibody

Purpose: Rab 5A Rabbit mAb

Specificity: This antibody detects endogenous levels of Rab 5A protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: Rab 5A

Background:

Synonyms: RAB5A, RAB5, Ras-related protein Rab-5A

Background: enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. , function:Required for the fusion of plasma membranes and early endosomes. , similarity:Belongs to the small GTPase superfamily. Rab family. , subcellular location:Enriched in stage I melanosomes. , subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. ,

Gene Name: >>Ras signaling pathway, >>Endocytosis, >>Phagosome, >>Vasopressin-regulated water reabsorption, >>Amyotrophic lateral sclerosis, >>Pathways of neurodegeneration-multiple diseases, >>Salmonella infection, >>Amoebiasis, >>Tuberculosis

Protein Name: Rab 5A

Gene ID: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity