RAD17 antibody (pSer635)

Catalog No. ABIN8124219

The Rabbit Polyclonal anti-RAD17 antibody is suitable to detect RAD17 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for RAD17 antibody (pSer635) (ABIN8124219)

Target: RAD17

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RAD17 Antibody

Binding Specificity: pSer635

Purpose: Rad17 (Phospho Ser635) Antibody

Specificity: This antibody detects endogenous levels of Human Rad17 (phospho-Ser635)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized phosho peptide around human Rad17 (Ser635)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RAD17

Target: RAD17

Alternative Name: Rad17

Background:

Synonyms: Cell cycle checkpoint protein RAD17 (hRad17) (RF-C/activator 1 homolog)

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

Gene Name: RAD17 R24L

Protein Name: Rad17 (Ser635)

Gene ID: 5884

UniProt: O75943