RAD17 antibody (pSer646)

Catalog No. ABIN8124220

The Rabbit Polyclonal anti-RAD17 antibody (ABIN8124220) specifically detects RAD17 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAD17 antibody (pSer646) (ABIN8124220)

Target: RAD17

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RAD17 Antibody

Binding Specificity: pSer646

Purpose: Rad17 (phospho Ser646) Antibody

Specificity: Phospho-Rad17 (S646) Polyclonal Antibody detects endogenous levels of Rad17 protein only when phosphorylated at S646.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Rad17 (phospho Ser646)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RAD17

Target: RAD17

Alternative Name: Rad17

Background:

Synonyms: RAD17, R24L, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

Gene Name: RAD17

Protein Name: Cell cycle checkpoint protein RAD17

Gene ID: 5884

UniProt: O75943