RAD50 antibody (pSer635)

Catalog No. ABIN8124222

The Rabbit Polyclonal anti-RAD50 antibody is suitable to detect RAD50 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for RAD50 antibody (pSer635) (ABIN8124222)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAD50 Antibody

Binding Specificity: pSer635

Purpose: Rad50 (Phospho Ser635) Antibody

Specificity: This antibody detects endogenous levels of Human Rad50 (phospho-Ser635)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized phosho peptide around human Rad50 (Ser635)

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: Rad50

Background:

Synonyms: DNA repair protein RAD50 (hRAD50) (EC 3.6.-.-)

Background: RAD50 double strand break repair protein(RAD50) Homo sapiens The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010],

Gene Name: RAD50

Protein Name: Rad50 (Ser635)

Gene ID: 10111

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus