RHOA antibody

Catalog No. ABIN8124244

The Rabbit Monoclonal anti-RHOA antibody (Clone AB1677) (ABIN8124244) specifically detects RHOA in WB, ELISA, IHC, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RHOA antibody (ABIN8124244)

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1677

Product Details anti-RHOA Antibody

Purpose: RhoA Rabbit mAb

Specificity: This antibody detects endogenous levels of RhoA protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA

Background:

Synonyms: Transforming protein RhoA (Rho cDNA clone 12) (h12)

Background: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],

Gene Name: RHOA ARH12 ARHA RHO12

Protein Name: RhoA

Gene ID: 387

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome