RNF144B antibody

Catalog No. ABIN8124438

The Rabbit Polyclonal anti-RNF144B antibody is suitable to detect RNF144B in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for RNF144B antibody (ABIN8124438)

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF144B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF144B Antibody

Purpose: p53RFP Antibody

Specificity: P53RFP Polyclonal Antibody detects endogenous levels of p53RFP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human p53RFP.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RNF144B

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Alternative Name: p53RFP

Background:

Synonyms: RNF144B, IBRDC2, P53RFP, E3 ubiquitin-protein ligase RNF144B, IBR domain-containing protein 2, RING finger protein 144B, p53-inducible RING finger protein

Background: caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification, protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.,

Gene Name: RNF144B

Protein Name: E3 ubiquitin-protein ligase RNF144B

Gene ID: 255488

UniProt: Q7Z419