SIRT3 antibody

Catalog No. ABIN8124717

The Rabbit Monoclonal anti-SIRT3 antibody is suitable to detect SIRT3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IP and IF.

Quick Overview for SIRT3 antibody (ABIN8124717)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: AB1803

Product Details anti-SIRT3 Antibody

Purpose: SIRT3 Rabbit mAb

Specificity: This antibody detects endogenous levels of SIRT3 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background:

Synonyms: SIRT3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008],

Gene Name: SIRT3

Protein Name: SIRT3

Gene ID: 23410

UniProt: Q9NTG7