SMAD5 antibody (pSer463, Ser465)

Catalog No. ABIN8124746

This Rabbit Monoclonal antibody specifically detects SMAD5 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SMAD5 antibody (pSer463, Ser465) (ABIN8124746)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1609

Product Details anti-SMAD5 Antibody

Binding Specificity: pSer463, Ser465

Purpose: SMAD5 (Phospho Ser463/Ser465) Rabbit mAb

Specificity: The antibody detects endogenous levels of SMAD5 protein only when phosphorylated at Ser463 and Ser465. The antibody does not cross-react with SMAD5 phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background:

Synonyms: SMAD5, MADH5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5

Background: The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Gene Name: SMAD5

Protein Name: SMAD5 (Phospho Ser463/Ser465)

Gene ID: 4090

UniProt: Q99717