Topoisomerase II alpha antibody (pSer1213)

Catalog No. ABIN8125249

The Rabbit Polyclonal anti-Topoisomerase II alpha antibody (ABIN8125249) specifically detects Topoisomerase II alpha in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Topoisomerase II alpha antibody (pSer1213) (ABIN8125249)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: pSer1213

Purpose: TOP2A (Phospho Ser1213) Antibody

Specificity: This antibody detects endogenous levels of Human TOP2A (Phospho Ser1213)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human TOP2A (Phospho Ser1213)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Synonyms: DNA topoisomerase 2-alpha (EC 5.99.1.3, DNA topoisomerase II, alpha isozyme)

Background: catalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,enzyme regulation:Specifically inhibited by the intercalating agent amsacrine.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylation has no effect on catalytic activity.,similarity:Belongs to the type II topoisomerase family.,subcellular location:Generally located in the nucleoplasm.,subunit:Homodimer. Interacts with COPS5.,

Gene Name: TOP2A TOP2

Protein Name: TOP2A (Phospho Ser1213)

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases