TCF7 antibody

Catalog No. ABIN8125271

This Rabbit Monoclonal antibody specifically detects TCF7 in WB, ELISA, IHC, IF and IP. It exhibits reactivity toward Human and Rat.

Quick Overview for TCF7 antibody (ABIN8125271)

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TCF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2126

Product Details anti-TCF7 Antibody

Purpose: TCF7 Rabbit mAb

Specificity: This antibody detects endogenous levels of TCF7 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for TCF7

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Alternative Name: TCF7

Background:

Synonyms: TCF7, TCF1, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1

Background: alternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist, function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4 (+) CD8 (+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. , induction:By TCF7L2 and CTNNB1. , sequence caution:Wrong choice of frame. , similarity:Belongs to the TCF/LEF family. , similarity:Contains 1 HMG box DNA-binding domain. , subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. , tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. ,

Gene Name: TCF7

Protein Name: TCF7

Gene ID: 6932

UniProt: P36402

Pathways: WNT Signaling