Vinculin antibody (AA 100-200)

Catalog No. ABIN8125716

The Mouse Monoclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for Vinculin antibody (AA 100-200) (ABIN8125716)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB2357

Product Details anti-Vinculin Antibody

Binding Specificity: AA 100-200

Purpose: Vinculin mouse mAb

Specificity: This antibody detects endogenous levels of Vinculin protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 100 - 200 of the human Vinculin protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background:

Synonyms: VCL, Vinculin, Metavinculin

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Gene Name: VCL

Protein Name: Vinculin

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2