Nitrilase 1 antibody (AA 111-210) (HRP)

Catalog No. ABIN762095

The Rabbit Polyclonal anti-Nitrilase 1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Nitrilase 1 in samples from Human.

Quick Overview for Nitrilase 1 antibody (AA 111-210) (HRP) (ABIN762095)

Target: Nitrilase 1 (NIT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrilase 1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Nitrilase 1 Antibody (HRP)

Binding Specificity: AA 111-210

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NIT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nitrilase 1

Target: Nitrilase 1 (NIT1)

Alternative Name: NIT1

Background:

Synonyms: NIT1, NIT1_HUMAN, Nitrilase 1, Nitrilase homolog 1.

Background: Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells, this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.

Gene ID: 4817