KDM5B antibody (AA 65-165) (HRP)

Catalog No. ABIN762995

Product Details anti-KDM5B Antibody (HRP)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Binding Specificity: AA 65-165

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5B antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KDM5B/Jarid1B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: KDM5B (KDM5B Products)

Synonyms: jarid1b antibody, jarid1ba antibody, id:ibd5050 antibody, wu:fd15c05 antibody, cb264 antibody, jarid1bb antibody, plu1 antibody, wu:fc44h11 antibody, zgc:85741 antibody, JARID1B antibody, CT31 antibody, PLU-1 antibody, PLU1 antibody, PUT1 antibody, RBBP2H1A antibody, 2010009J12Rik antibody, 2210016I17Rik antibody, AW556288 antibody, D1Ertd202e antibody, Jarid1b antibody, Plu1 antibody, Rb-Bp2 antibody, mKIAA4034 antibody, RGD1565602 antibody, lysine (K)-specific demethylase 5Ba antibody, lysine (K)-specific demethylase 5Bb antibody, lysine demethylase 5B antibody, lysine (K)-specific demethylase 5B antibody, kdm5ba antibody, kdm5bb antibody, KDM5B antibody, Kdm5b antibody

Background:

Synonyms: CT31, PLU1, PUT1, PLU-1, JARID1B, PPP1R98, RBBP2H1A, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1, KDM5B, RBBP2H1

Background: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

Gene ID: 10765

UniProt: Q9UGL1

Pathways: Warburg Effect