NLRP2 antibody (C-Term)

Catalog No. ABIN768539

The Goat Polyclonal anti-NLRP2 antibody has been validated for WB and ELISA. It is suitable to detect NLRP2 in samples from Human.

Quick Overview for NLRP2 antibody (C-Term) (ABIN768539)

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NLRP2 Antibody

Binding Specificity: C-Term

Purpose: NALP2 / NLRP

Sequence: TEKHHPWAER PSSHD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TEKHHPWAERPSSHD, from the C Terminus of the protein sequence according to NP_060322.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx. 150 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NLRP2

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Alternative Name: NLRP2

Background: NLRP2, NLR family, pyrin domain containing 2, CLR19.9, FLJ20510, NALP2, NBS1, PAN1, PYPAF2, NACHT, LRR and PYD containing protein 2, NACHT, leucine rich repeat and PYD containing 2, PYRIN-Containing APAF1-like, nucleotide-binding oligomerization domain, l

Molecular Weight: 150kDa

Gene ID: 55655

NCBI Accession: NP_060322

Pathways: Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome