IDH3G antibody (AA 337-350)

Catalog No. ABIN768559

This Goat Polyclonal antibody specifically detects IDH3G in ELISA, WB and IHC. It exhibits reactivity toward Human, Rat and Pig.

Quick Overview for IDH3G antibody (AA 337-350) (ABIN768559)

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Reactivity: Human, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH3G antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-IDH3G Antibody

Binding Specificity: AA 337-350

Purpose: IDH3G (aa337-350)

Sequence: DHLKLHSYAT SIRK

Specificity: This antibody is expected to recognize both reported isoforms (NP_004126.1, NP_777358.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DHLKLHSYATSIRK, from the internal region of the protein sequence according to NP_004126.1, NP_777358.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 38 kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5 kDa according to NP_777358.1). Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH3G

Target: IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))

Alternative Name: IDH3G

Background: IDH3G, isocitrate dehydrogenase 3 (NAD+) gamma, H-IDHG, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, OTTHUMP00000025984, OTTHUMP00000025987, OTTHUMP00000025988, isocitrate dehy

Molecular Weight: 41.5kDa according to NP_777358.1

Gene ID: 3421, 15929, 25179

NCBI Accession: NP_004126, NP_777358