SOD2 antibody (Internal Region)

Catalog No. ABIN768567

Product Details anti-SOD2 Antibody

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: MNSOD (isoform A)

Sequence: DVTAQIALQP ALKFN

Specificity: This antibody is expected to recognize isoform A (NP_000627.2) only. Reported variants represent identical protein: NP_000627.2, NP_001019636.1.

Cross-Reactivity: Cow, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DVTAQIALQPALKFN, from the internal region of the protein sequence according to NP_000627.2,.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 5 μg/mL.

Western Blot: Approx 23 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex) and HeLa lysates (calculated MW of 24.8 kDa according to NP_000627.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: SOD2 (SOD2 Products)

Background: SOD2, superoxide dismutase 2, mitochondrial, RP1-56L9.2, IPOB, MNSOD, MVCD6, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase [Mn], mitochondrial

Molecular Weight: 24.8kDa according to NP_000627.2

Gene ID: 6648

NCBI Accession: NP_000627

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling