SIRT3 antibody (Internal Region)

Catalog No. ABIN768568

This Goat Polyclonal antibody specifically detects SIRT3 in WB, ELISA, IF and FACS. It exhibits reactivity toward Mouse.

Quick Overview for SIRT3 antibody (Internal Region) (ABIN768568)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SIRT3 Antibody

Binding Specificity: Internal Region

Purpose: Sirtuin 3 (mouse)

Sequence: RASGIPASKL VEAH

Specificity: This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: Sirt3

Background: Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3

Molecular Weight: 28.8kDa according to NP_071878.2

Gene ID: 23410, 64384, 293615

NCBI Accession: NP_071878, NP_001171275