CHRNA5 antibody (Internal Region)

Catalog No. ABIN768569

The Goat Polyclonal anti-CHRNA5 antibody is suitable to detect CHRNA5 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for CHRNA5 antibody (Internal Region) (ABIN768569)

Target: CHRNA5 (Cholinergic Receptor, Nicotinic, alpha 5 (Neuronal) (CHRNA5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CHRNA5 Antibody

Binding Specificity: Internal Region

Purpose: CHRNA5

Sequence: HVDRYFTQKE ET

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HVDRYFTQKEET, from the internal region of the protein sequence according to NP_000736.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon (submucosal plexus). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell line KELLY (calculated MW of 53.1 kDa according to NP_000736.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHRNA5

Target: CHRNA5 (Cholinergic Receptor, Nicotinic, alpha 5 (Neuronal) (CHRNA5))

Alternative Name: CHRNA5

Background: CHRNA5, cholinergic receptor, nicotinic, alpha 5, LNCR2, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, neuronal acetylcholine receptor subunit alpha-5, neuronal nicotinic acetylcholine receptor, alpha5 subunit

Molecular Weight: 53.1kDa according to NP_000736.2

Gene ID: 1138

NCBI Accession: NP_000736