KEAP1 antibody (AA 41-53)

Catalog No. ABIN768571

This Goat Polyclonal antibody specifically detects KEAP1 in ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for KEAP1 antibody (AA 41-53) (ABIN768571)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-KEAP1 Antibody

Binding Specificity: AA 41-53

Purpose: KEAP1 (aa41-53)

Sequence: EVTPSQHGNR TFS

Specificity: Reported variants represent identical protein: NP_036421.2, NP_987096.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EVTPSQHGNRTFS, from the internal region (near N Terminus) of the protein sequence according to NP_036421.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration: 3-5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19

Molecular Weight: 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1

Gene ID: 9817, 50868, 117519

NCBI Accession: NP_036421

Pathways: Maintenance of Protein Location