UNC13B antibody (Internal Region)

Catalog No. ABIN768594

This anti-UNC13B antibody is a Goat Polyclonal antibody detecting UNC13B in ELISA and IHC. Suitable for Human.

Quick Overview for UNC13B antibody (Internal Region) (ABIN768594)

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC13B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UNC13B Antibody

Binding Specificity: Internal Region

Purpose: Unc13b (mouse)

Sequence: PLRDVAAKGS C

Specificity: This antibody is expected to recognize all reported isoforms (NP_074053.1, NP_001036044.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PLRDVAAKGSC, from the internal region of the protein sequence according to NP_074053.1, NP_001036044.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 110 kDa band in Rat Lung lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 184 kDa ac

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UNC13B

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Alternative Name: Unc13b

Background: Unc13b, unc-13 homolog B (C. elegans), Munc13-2, Unc13h2, unc-13 homolog B

Molecular Weight: 110kDa

Gene ID: 22249, 64830

NCBI Accession: NP_074053, NP_001036044

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis