Caspase 3 antibody (Internal Region)
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- Target See all Caspase 3 (CASP3) Antibodies
- Caspase 3 (CASP3)
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Caspase 3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Purpose
- Caspase 3
- Sequence
- RDVSKEDHSK RS
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2, NP_116786.1.
- Isotype
- IgG
- Top Product
- Discover our top product CASP3 Primary Antibody
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- Application Notes
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Western Blot: Approx 32 kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6 kDa according to Human NP_004337.2 and 31.4 kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65 kDa was also
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).
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Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).
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- Target
- Caspase 3 (CASP3)
- Alternative Name
- CASP3 (CASP3 Products)
- Synonyms
- CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody
- Background
- CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3
- Molecular Weight
- 31.6kDa according to NP_004337.2
- Gene ID
- 836
- NCBI Accession
- NP_004337, NP_116786
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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