IDH1 antibody (Internal Region)

Catalog No. ABIN768654

The Goat Polyclonal anti-IDH1 antibody (ABIN768654) specifically detects IDH1 in WB and ELISA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for IDH1 antibody (Internal Region) (ABIN768654)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Saccharomyces cerevisiae

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IDH1 Antibody

Binding Specificity: Internal Region

Purpose: IDH1 (yeast)

Sequence: EPGSRHVGLD IKGQN

Cross-Reactivity: Saccharomyces cerevisiae

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EPGSRHVGLDIKGQN, from the internal region of the protein sequence according to NP_014361.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3 kDa according to NP_014361.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background: IDH1, Idh1p, YNL037C, Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle

Molecular Weight: 39.3kDa according to NP_014361.1

NCBI Accession: NP_014361

Pathways: Warburg Effect