C3AR1 antibody (AA 63-8) (Biotin)

Catalog No. ABIN782445

This anti-C3AR1 antibody is a Mouse Monoclonal antibody detecting C3AR1 in FACS and IHC (fro). Suitable for Rat.

Quick Overview for C3AR1 antibody (AA 63-8) (Biotin) (ABIN782445)

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3AR1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 74

Product Details anti-C3AR1 Antibody (Biotin)

Binding Specificity: AA 63-8

Specificity: This rat C3a receptor antibody detects a recombinant peptide corresponding to amino acids 161-321 of the large extracellular loop structure, which shares 34 % homology with mouse and human. Deduced amino acid sequences of human/rat C3aR is 58.5 % and 90.7 % with rat/mouse.

Purification: Affinity chromatography on Protein G

Immunogen: RBL-2H3 transfectants expressing rat C3aR Donor: BALB/c Fusion Partner: X63-Ag8.653

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C3AR1

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Background: C3aR binds to anaphylotoxin C3a which, among other things, is involved in mast cell degranulation and recruitment of immune cells to the site of inflammation. Activation of the complement cascade produces various fragments, including C3a and C5a. C3aR is characterized by seven transmembrane domains including a large extracellular loop which is coupled to a Gi protein. C3aR expression is detected on glial cells, neurons and cells belonging to the mononuclear phagocyte system. C3aR expression was shown to moderately increase after LPS stimulation.Synonyms: AZ3B, C3AR, C3AR1, C3R1, C3a anaphylatoxin chemotactic receptor, C3a-R, HNFAG09

Gene ID: 84007

NCBI Accession: NP_114449

UniProt: O55197

Pathways: Complement System