IL-5 antibody

Catalog No. ABIN783054

The Rabbit Polyclonal anti-IL-5 antibody has been validated for WB and EIA. It is suitable to detect IL-5 in samples from Mouse.

Quick Overview for IL-5 antibody (ABIN783054)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-5 Antibody

Specificity: Recognizes Interleukin-5/IL5.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography employing an immobilized Murine IL-5 matrix

Immunogen: Highly pure recombinant Murine IL-5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin-5 / IL5

Background: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

Gene ID: 16191

NCBI Accession: NP_034688

UniProt: P04401

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour