PKM2 antibody (AA 399-403)

Catalog No. ABIN783340

This Rabbit Polyclonal antibody specifically detects PKM2 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for PKM2 antibody (AA 399-403) (ABIN783340)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKM2 Antibody

Binding Specificity: AA 399-403

Sequence: V-R-A-S-S

Specificity: This antibody detects endogenous levels of total PKM1 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse

Purification: Affinity-chromatography using epitope-specific peptide

Immunogen: Peptide sequence around aa. 399~403

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

Molecular Weight: 60 kDa

Gene ID: 5315

NCBI Accession: NP_002645

UniProt: P14618

Pathways: Warburg Effect