GLS2 antibody (Center)

Catalog No. ABIN783413

The Rabbit Polyclonal anti-GLS2 antibody has been validated for WB and EIA. It is suitable to detect GLS2 in samples from Human, Mouse and Rat.

Quick Overview for GLS2 antibody (Center) (ABIN783413)

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLS2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GLS2 Antibody

Binding Specificity: Center

Specificity: This antibody detects Glutaminase liver isoform at Center. GLS2 antibody is predicted to not cross-react with other catenin family members. Multiple isoforms of GLS2 are known to exist.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 18 amino acid peptide near the center terminus of human GLS2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Background: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and ?-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.Synonyms: GA, GLS2, L-glutaminase, L-glutamine amidohydrolase

Gene ID: 27165

NCBI Accession: NP_037399

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, Warburg Effect