NUMB antibody (C-Term)

Catalog No. ABIN783468

The Rabbit Polyclonal anti-NUMB antibody (ABIN783468) specifically detects NUMB in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NUMB antibody (C-Term) (ABIN783468)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NUMB Antibody

Binding Specificity: C-Term

Specificity: This antibody detects NUMB at C-term. At least four isoforms of NUMB are known to exist, this antibody will detect all of them.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 17 amino acid peptide near the carboxy terminus of human NUMB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells (1,2). NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.Synonyms: Protein S171, Protein numb homolog, h-Numb

Gene ID: 8650

NCBI Accession: NP_001005743

UniProt: P49757

Pathways: Cell-Cell Junction Organization