TRIM5 antibody (C-Term, Isoform gamma)

Catalog No. ABIN783538

This Rabbit Polyclonal antibody specifically detects TRIM5 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for TRIM5 antibody (C-Term, Isoform gamma) (ABIN783538)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TRIM5 Antibody

Binding Specificity: C-Term, Isoform gamma

Specificity: This antibody detects TRIM5 / RNF88 (Isoform gamma) at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography purified via peptide column

Immunogen: 15 amino acids near the carboxy-terminus of human TRIM5 gamma

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5 / RNF88

Background: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). While a function has not yet been assigned to TRIM5γ, it is known that expression of TRIM5γ variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. Furthermore, the TRIM5γ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).Synonyms: RING finger protein 88, Tripartite motif-containing protein 5

Gene ID: 85363

NCBI Accession: NP_149023

UniProt: Q9C035

Pathways: Activation of Innate immune Response