ATG13 antibody (C-Term)

Catalog No. ABIN783663

The Rabbit Polyclonal anti-ATG13 antibody (ABIN783663) specifically detects ATG13 in WB, IF and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ATG13 antibody (C-Term) (ABIN783663)

Target: ATG13 (Autophagy Related 13 (ATG13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG13 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ATG13 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 15 amino acid peptide near the carboxy terminus of human ATG13

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Alternative Name: KIAA0652

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions, inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.Synonyms: UPF0630 protein KIAA0652

Gene ID: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: PI3K-Akt Signaling, Autophagy