ATG101 antibody (Center)

Catalog No. ABIN783665

The Rabbit Polyclonal anti-ATG101 antibody is suitable to detect ATG101 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p), IF and EIA.

Quick Overview for ATG101 antibody (Center) (ABIN783665)

Target: ATG101 (C12orf44) (Chromosome 12 Open Reading Frame 44 (C12orf44))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG101 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ATG101 Antibody

Binding Specificity: Center

Specificity: This antibody recognizes ATG101 and is predicted to not cross-react with other TG members.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity Chromatography purified via peptide column

Immunogen: 16 amino acid peptide near the center of Human ATG101

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Antibody is supplied in PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATG101

Target: ATG101 (C12orf44) (Chromosome 12 Open Reading Frame 44 (C12orf44))

Alternative Name: ATG101

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions, inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.Synonyms: Autophagy-related protein 101, C12orf44, PP894, UPF0453 protein C12orf44

Gene ID: 60673

NCBI Accession: NP_001092143

UniProt: Q9BSB4

Pathways: Autophagy